Reporter

Part:BBa_K676013:Design

Designed by: Meng Li   Group: iGEM11_UCL_London   (2011-09-19)

Mu/Spy Ligation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1203


Design Notes

Performed 3A Assembly between the Mu phage GBS (Part:BBa_K676001) with the Spy promoter device (Part:BBa_K239009) constructed by iGEM09_UCL_London.

Source

Part:BBa_K676001 - iGEM11_UCL_London

Part:BBa_K239009 - iGEM09_UCL_London

References